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Antibodies bound to the blocking peptide no more bind to the epitope about the target protein. By evaluating the staining through the blocked antibody as opposed to the antibody alone, one can see which staining is distinct.

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This manual outlines additional possible optimization of cellular and immunoassay parameters to ensure the best possible outcomes are...

This tutorial outlines further feasible optimization of mobile and immunoassay parameters to be sure the very best final results are...

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If an Invitrogen™ antibody won't perform as described on our Web site or datasheet,we will switch the solution at no cost to you personally, or provide you with a credit for the foreseeable future order.*

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Clicking the pictures or links will redirect you to definitely a website hosted by BenchSci that gives 3rd-get together scientific content. Neither the written content nor the BenchSci technology and processes for range have already been evaluated by us; we're providing them as-is and devoid of warranty of any form, which includes to be used or application from the Thermo Fisher Scientific goods offered.

The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by both equally antibodies...

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Blocking peptides are peptides that bind precisely to your concentrate on antibody and block antibody binding. These peptide generally contains the epitope acknowledged because of the antibody.

Concentrate on Information and facts Cyclin E belongs to the highly conserved cyclin spouse and children, whose associates are characterised by a spectacular periodicity in protein abundance throughout the cell cycle. Cyclins purpose as regulators of CDK kinases. Unique cyclins show unique expression and degradation designs which contribute into the temporal coordination of every mitotic occasion. Cyclin E kinds a complex with and functions for a regulatory subunit of CDK2, whose activity is needed for cell cycle G1/S transition. This protein accumulates for the G1-S period boundary and is degraded as cells development through S stage.

Overexpression of the gene has become observed in lots of tumors, which results in chromosome instability, and so may well contribute to tumorigenesis. This protein was found to affiliate with, and be associated with, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in cell-cycle regulated histone gene expression and performs a vital purpose in advertising cell-cycle progression while in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are already explained. Two supplemental splice variants had been described but specific nucleotide sequence information is just not however available.

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